Human Gamma Globulin Fractionation on Anion Exchange Cellulose Columns

Human gamma globulin fractionation on anion exchange cellulose columns.

The serum proteins designated as y-globulins include all proteins of the slowest moving of the several major protein groups seen on serum electrophoresis conducted at alkaline pH (2). Among the y-globulins of normal serum are proteins of differing electrophoretic mobility (3,4), proteins with sedimentation coefficients of 6.6 S and 18 S in the ultracentrifuge (2, 3, 5, 6)) many individual antib...

The fractionation of urine colloids on anion-exchange cellulose.

Thyroglobulin fractionation on diethylaminoethyl cellulose columns.

Thyroglobulin may be defined as the iodine-containing protein of the thyroid gland which has a sedimentation coefficient of 4o.w = 19 S, an electrophoretic mobility at pH 8.6 of approximately 5 x 10F5 cm2 volt-l set-1, and a steep salting out curve at about 38% ammonium sulfate saturation at neutral pH (1). Although other iodoproteins, both soluble in neutral salt and attached to cell particles...

Human Gamma Globulin.

FRACTIONATION OF HUMAN IMMUNE r-GLOBULIN

Equine and bovine serum proteins have recently been fractionated by means of a physical method utilizing an electrophoretic adaptation of the principles of the Clusius column (l-4), first described and tested by Kirkwood (5) and Nielsen (6). The method of electrophoresis-convection has now been applied to the fractionation of human r-globulin. The r-globulin was prepared by ethanol fractionatio...